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Updated: Jun 20, 2026

A Competent Hepatocyte Model Examining Hepatitis B Virus Entry through Sodium Taurocholate Cotransporting Polypeptide as a Therapeutic Target
Published on: May 10, 2022
Tryptophan and iodothyronine transport interactions in HepG2 human hepatoma cells
James W A Ritchie1, Peter Maving Taylor
1Division of Molecular Physiology, College of Life Sciences, James Black Centre, University of Dundee, Dundee, DD1 5EH, UK.
Abstract:
This study identifies interactions between transport of the aromatic amino acid L: -tryptophan (Trp) and thyroid hormones (TH) in HepG2 human hepatoma cells. The major portion of Trp uptake in HepG2 cells occurs via the NEM-sensitive amino acid transport System L2 (consistent with hepatic LAT3 expression), with a smaller aromatic-AA selective System T (MCT10) component. LAT3 and MCT10 mRNA were both detected in HepG2 cells. Uptake of TH does not involve System L2, but a significant portion of T(3) uptake is mediated by System T, alongside a taurocholate-sensitive organic anion transporter. T(4) uptake into HepG2 cells appears to be mediated principally by organic anion/monocarboxylate transporters, with smaller contributions by System T and receptor-mediated endocytosis. TH-Trp transport interactions in liver cells centre on System T which, due to a perivenous localisation alongside deiodinase 1, may impact on hepatic T(3) generation and release.
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