Individual identification of human bloodstains investigated with hypervariable DNA loci

Seven kinds of hypervariable DNA probes, which recognize hypervariable loci, were applied for individual identification of human bloodstains. Allelic frequencies and their confidence intervals among unrelated Japanese individuals were previously reported from our laboratory, and codominant segregation of the polymorphism was confirmed in family studies. One-month-old bloodstains from ten individuals were investigated in the present study. The probability of matching was calculated using the database among Japanese population. Cumulative probability of matching from 7 kinds of hypervariable DNA probes was 1.1 x 10(-11), which was 4.5 x 10(9) times higher than that from 6 kinds of common blood group markers such as ABO, MN, Rh-Hr, P1, Hp and PGM1. Accordingly, DNA polymorphism is considered to be informative enough for individual identification of bloodstains.