Related Experiment Videos
Unique sequences for two HLA-DRB1 genes expressed on distinct DRw6 haplotypes
E W Petersdorf1, R L Griffith, H A Erlich
1Division of Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
Immunogenetics
|January 1, 1990
Summary
Two novel human leukocyte antigen (HLA) DRB1 alleles, "HAG" and "PEV", were identified using DNA sequencing and restriction fragment length polymorphism (RFLP) analysis. These alleles expand our understanding of DRw52-associated haplotypes and their diversity.
Area of Science:
- Immunogenetics
- Molecular Biology
- Human Leukocyte Antigen (HLA) System
Background:
- Conventional human leukocyte antigen (HLA) serology has limitations in defining specific alleles.
- DRw52 and DQw7-associated haplotypes present unique challenges for precise genetic characterization.
Purpose of the Study:
- To characterize two distinct DRB1 alleles, designated HLA-D "HAG" and "PEV", associated with DRw52 and DQw7 haplotypes.
- To elucidate the genetic basis and evolutionary origins of these novel alleles using molecular techniques.
Main Methods:
- Restriction fragment length polymorphism (RFLP) analysis was employed to examine variations in DNA.
- DNA sequencing of the DRB1 genes was performed to determine the precise genetic makeup of the "HAG" and "PEV" haplotypes.
Main Results:
- Two novel DRB1 alleles, "HAG" (DRB1*1303) and "PEV", were identified, which are not easily defined by standard DR serology.
- "HAG" and "PEV" are associated with different DRw52 variants, with "HAG" showing similarities to DRw18(3) haplotypes.
- Sequence analysis suggests "HAG" arose from recombination and/or mutation events, while "PEV" is a recombinant allele of DRB1*1301-1302 and DRB1*1101.
Conclusions:
- The DRw52 family of haplotypes likely originates from a limited set of ancestral sequences.
- Genetic diversity within DRB1 alleles of the DRw52 family is generated through mechanisms such as gene conversion and recombination.