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Updated: Jun 20, 2026

Area-based Image Analysis Algorithm for Quantification of Macrophage-fibroblast Cocultures
Published on: February 15, 2022
Nuno Vieira Lopes1, Pedro A Mogadouro do Couto, Humberto Bustince
1Department of Electrical Engineering, Escola Superior de Tecnologia e Gestão-IPL, Campus 1, Apartado 4045, 2411-901 Leiria, Portugal. nlopes@estg.ipleiria.pt
This study introduces an improved automatic histogram thresholding method using fuzzy logic. It enhances image segmentation by analyzing gray-level similarity for optimal threshold determination without prior image knowledge.
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