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T Cell Types and Functions

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Related Experiment Video

Updated: Jun 20, 2026

Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer's Patches Using Adoptive Transfer of Hematopoietic Progenitors

Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer's Patches Using Adoptive Transfer of Hematopoietic Progenitors

Published on: March 17, 2014

Modulation of human plasmacytoid DC function by IFN-lambda1 (IL-29).

Nicholas J Megjugorac¹, Grant E Gallagher, Grant Gallagher

¹Genetic Immunology Laboratory, HUMIGEN LLC, Institute for Genetic Immunology, Hamilton, New Jersey 08690, USA.

Journal of Leukocyte Biology

|September 18, 2009

Summary

Type III interferons (IFNs) impact immune cells. This study shows IFN-lambda1 influences plasmacytoid dendritic cell (pDC) activation and their ability to stimulate cytokine production, highlighting a role in immune responses.

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Development and Functional Characterization of Murine Tolerogenic Dendritic Cells

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Area of Science:

Immunology
Virology
Cell Biology

Background:

Type III interferons (IFNs) possess immunomodulatory and antiviral properties.
IFN-lambda members signal through a common receptor complex involving IL-28Ralpha and IL-10Rbeta.
The precise effects of IFN-lambda on diverse immune cell subsets remain incompletely understood.

Purpose of the Study:

To investigate the role of IFN-lambda in the maturation and development of plasmacytoid dendritic cells (pDCs).
To characterize the impact of IFN-lambda1 on pDC surface molecule expression and immunostimulatory potential.

Main Methods:

Quantification of IL-28Ralpha mRNA in peripheral blood pDCs.
Stimulation of pDCs with Herpes Simplex Virus (HSV) or Imiquimod.
Analysis of pDC surface molecule expression (CD80, ICOS-L, CD83, homing molecules) following IFN-lambda1 treatment.
Assessment of IFN-lambda1-treated pDC's ability to stimulate cytokine production (IL-13, IFN-gamma, IL-10) in mixed lymphocyte reactions (MLRs).

Main Results:

High levels of IL-28Ralpha mRNA were detected in pDCs, supporting a role for IFN-lambda.
pDC stimulation with HSV or Imiquimod increased IL-28Ralpha mRNA expression.
IFN-lambda1 modulated costimulatory molecules (CD80, ICOS-L) and synergized with IFN-alpha to up-regulate CD83 on pDCs.
IFN-lambda1 treatment altered homing molecule expression on pDCs and myeloid dendritic cells (mDCs).
IFN-lambda1-treated pDCs exhibited distinct cytokine production profiles (IL-13, IFN-gamma, IL-10) in MLRs.

Conclusions:

IFN-lambda signaling is relevant to pDC function.
IFN-lambda1 plays a significant role in pDC activation, influencing their surface phenotype and immunostimulatory capacity.
This study elucidates the variable effects of IFN-lambda on dendritic cell subsets and identifies a novel function in pDC activation.