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Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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Updated: Jun 20, 2026

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping

Published on: May 25, 2015

The 'PREXCEL-Q Method' for qPCR.

Jack M Gallup¹, Mark R Ackermann

¹Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA.

International Journal of Biomedical Science : IJBS

|September 18, 2009

Summary

This summary is machine-generated.

This study introduces PREXCEL-Q (P-Q), a software tool for quantitative real-time polymerase chain reaction (qPCR) assay development and project management. P-Q ensures reliable, efficient, and uninhibited qPCR reactions for any sample type.

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Area of Science:

Molecular Biology
Biotechnology
Bioinformatics

Background:

Quantitative real-time polymerase chain reaction (qPCR) is a vital technique in molecular biology.
Assay development and project management for qPCR can be complex and prone to errors.
Standardized and reliable methods are needed to optimize qPCR experimental design and execution.

Purpose of the Study:

To describe a reliable approach to quantitative real-time polymerase chain reaction (qPCR) assay development and project management.
To introduce the PREXCEL-Q (P-Q) software, designed to facilitate this process.
To highlight the program's ability to address common qPCR challenges.

Main Methods:

Development and utilization of the PREXCEL-Q (P-Q) software tool.
Addressing sample-related inhibitory problems, concentration limitations, and nuclease treatment.
Incorporating reverse transcription (RT) and master mix formulation considerations.

Main Results:

PREXCEL-Q enables investigators to design qPCR reactions that are uninhibited and dynamically sound.
The software facilitates LOG-linear amplification and high efficiency across all qPCR applications.
The program can manage an infinite number of samples, offering scalability.

Conclusions:

PREXCEL-Q provides a unique and defined approach to qPCR assay development and project management.
The software empowers researchers to achieve consistent, confident, and efficient qPCR results.
PREXCEL-Q is a valuable tool for optimizing qPCR experiments and overcoming common experimental hurdles.