Related Experiment Video
Updated: Aug 13, 2026

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations
Published on: November 3, 2010
Two chromosomal locations for human ornithine decarboxylase gene sequences and elevated expression in colorectal
D M Radford1, H Nakai, R L Eddy
1Department of Surgical Oncology, Roswell Park Memorial Institute, New York State Department of Health, Buffalo 14263.
Abstract:
The polyamines are known to be essential for cellular proliferation. Ornithine decarboxylase (ODC) is a rate-limiting enzyme in the synthesis of these amines, and activity is elevated in colorectal tumors and polyps. Two ODC genes (designated ODC1 and ODC2) were localized by somatic cell hybridization and in situ techniques to 2p25 and 7q31-qter, respectively. Investigation of the expression of ODC in colorectal neoplasia reveals a consistent increase in mRNA expression compared with normal adjacent mucosa and control mucosa, ranging from 1.3- to 12.2-fold. No amplification of the loci was seen. Comparison of ODC mRNA expression with ODC activity from the same samples revealed no direct correlation, suggesting that regulation of ODC in this system occurs at the posttranscriptional level.
Related Concept Videos
Epigenetic Regulation
Chromatin Position Affects Gene Expression
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the timing and level of...
Non-LTR Retrotransposons
Cell Specific Gene Expression
Exon Recombination
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Epigenetic Regulation
X-chromosome...

