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Parentage analysis by endonuclease shattering of hypervariable DNA.

F A Chiafari¹, R E Wenk

¹Baltimore Rh Typing Laboratory, Inc., Maryland.

|September 1, 1990

Summary

DNA fingerprinting using restriction enzymes can reveal more than just fragment length. A paternity case highlights how using a second enzyme, Hae III, can differentiate between similar DNA fragments and exclude a potential father.

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Area of Science:

Molecular Biology
Forensic Genetics
Biochemistry

Background:

Parentage testing commonly employs single-locus DNA probes targeting tandem repeat sequences and restriction enzymes to analyze heritable restriction fragment lengths.
Traditional Southern blot analysis using DNA probes reveals only fragment length and complementarity to the probe, potentially masking subtle genetic variations.

Observation:

Not all restriction fragments of identical apparent length are truly identical; differences can exist within the nucleotide sequences.
Using restriction enzymes that recognize subsets of repeat sequences increases the information content of DNA loci by detecting these differences.

Findings:

In a paternity case, initial DNA phenotyping with Hinf I suggested two brothers could be the father.
A subsequent DNA phenotyping analysis using a different restriction enzyme, Hae III, successfully excluded one of the brothers.

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Implications:

Employing multiple restriction enzymes enhances the discriminatory power of DNA fingerprinting in forensic and parentage testing.
This approach allows for the detection of variations in comigrating DNA fragments, increasing the accuracy of genetic analyses.