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Updated: Jun 20, 2026

Preparation of Single-cell Suspensions for Cytofluorimetric Analysis from Different Mouse Skin Regions
Published on: April 20, 2016
Skin immune sentinels in health and disease
Frank O Nestle1, Paola Di Meglio, Jian-Zhong Qin
1St. John's Institute of Dermatology, King's College London, UK. frank.nestle@kcl.ac.uk
Abstract:
Human skin and its immune cells provide essential protection of the human body from injury and infection. Recent studies reinforce the importance of keratinocytes as sensors of danger through alert systems such as the inflammasome. In addition, newly identified CD103(+) dendritic cells are strategically positioned for cross-presentation of skin-tropic pathogens and accumulating data highlight a key role of tissue-resident rather than circulating T cells in skin homeostasis and pathology. This Review focuses on recent progress in dissecting the functional role of skin immune cells in skin disease.
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