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Updated: Jun 20, 2026

A Sensitive and Specific Quantitation Method for Determination of Serum Cardiac Myosin Binding Protein-C by Electrochemiluminescence Immunoassay
Published on: August 8, 2013
Metal-enhanced fluorescent biomarkers for cardiac risk assessment
Evgenia Matveeva1, Zygmunt Gryczynski, Joanna Malicka
1Center for Fluorescence Spectroscopy, University of Maryland at Baltimore Medical School, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, MD 21201.
Abstract:
In this presentation we describe a novel methodology for ultra-sensitive fluorescence immunoassays based on a new class of fluorescent biomarkers, which are strongly enhanced by nano-size metallic particles. Specifically, we discuss development of the immunoassay on the surfaces coated with metallic particles for high sensitivity detection of cardiac markers. This technology will allow detection of the biomarkers in serum and blood without separation and amplification steps. We present an experimental platform that uses front-face excitation in total internal reflection mode for efficient rejection of background fluorescence.
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