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Related Concept Videos

Positive Regulator Molecules02:39

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
Positive Regulator Molecules01:45

Positive Regulator Molecules

To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
The Cell Cycle Control System02:11

The Cell Cycle Control System

The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
The Cell Cycle Control System01:28

The Cell Cycle Control System

The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Cells Coordinate Growth and Proliferation02:36

Cells Coordinate Growth and Proliferation

Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...

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Related Experiment Video

Updated: Jun 20, 2026

Analysis of Cell Cycle Position in Mammalian Cells
12:19

Analysis of Cell Cycle Position in Mammalian Cells

Published on: January 21, 2012

Cell cycle-related cyclin b1 quantification.

Phyllis S Frisa1, James W Jacobberger

  • 1Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, Ohio, USA.

Plos One
|September 19, 2009
PubMed
Summary
This summary is machine-generated.

This study quantifies cyclin B1 protein levels in human cells using Western blotting and cytometry. Results show cyclin B1 expression peaks similarly across cell lines, suggesting tight control of cell cycle progression.

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Last Updated: Jun 20, 2026

Analysis of Cell Cycle Position in Mammalian Cells
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Published on: January 21, 2012

Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations
10:54

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Published on: September 17, 2012

Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry
11:23

Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry

Published on: May 22, 2012

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Accurate quantification of intracellular proteins is crucial for understanding cellular processes.
  • Previous work established methods for quantifying SV40 large T antigen using Western blots and cytometry.
  • A modified approach is presented for quantifying endogenous cyclin B1 and its cell cycle-dependent expression.

Purpose of the Study:

  • To quantify endogenous cyclin B1 levels in human cells.
  • To generate a cell cycle time-related expression profile for cyclin B1.
  • To establish a reliable method for correlating Western blot and cytometry data for protein quantification.

Main Methods:

  • Purification of recombinant cyclin B1 using antibody affinity chromatography.
  • Preparation of fixed cell samples (lyophilized and solubilized) from PC3 cells with varying cyclin B1 levels.
  • Western blotting and cytometry of purified cyclin B1, cell preparations, and untreated cell lines (K562, HeLa, RKO).

Main Results:

  • A linear correlation (r² = 0.87) was established between Western blot (molecules/cell) and cytometry (fluorescence) for cyclin B1.
  • Average cyclin B1 levels per cell ranged from 1.5 x 10⁵ to 2.5 x 10⁶ molecules, with a 12-fold variation in fluorescence.
  • Peak cyclin B1 levels in G2 phase were consistent across different cell lines, despite variations in growth conditions and accumulation rates.

Conclusions:

  • Cyclin B1 expression initiates in G1 phase and increases non-linearly throughout the cell cycle.
  • Peak cyclin B1 levels in G2 are tightly regulated and similar across diverse cell lines.
  • This suggests a robust quantitative control mechanism for cell cycle progression.