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Updated: Jun 20, 2026
Fluorescence-Based Detection of FEN1 Nuclease Activity and Screening of Small-Molecule Inhibitors
Published on: June 27, 2025
A new assay format for NF-kappaB based on a DNA triple helix and a fluorescence resonance energy transfer
Dominik Altevogt1, Andrea Hrenn, Claudia Kern
1Institut für Organische Chemie und Biochemie, Albert-Ludwigs-Universität Freiburg, Albertstr. 21, Freiburg, 79104, Germany.
Abstract:
Herein we report a feasibility study for a new concept to detect DNA binding protein NF-kappaB based on a DNA triple helix formation in combination with a fluorescence resonance energy transfer (FRET). The new principle avoids expensive antibodies and radioactivity and might have implications for assays of other DNA binding proteins.
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