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Related Concept Videos

Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...
Regulated mRNA Transport02:22

Regulated mRNA Transport

In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...

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Related Experiment Video

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Evaluation of the Impact of Protein Aggregation on Cellular Oxidative Stress in Yeast
11:04

Evaluation of the Impact of Protein Aggregation on Cellular Oxidative Stress in Yeast

Published on: June 23, 2018

Correlation between mRNA expression levels and protein aggregation propensities in subcellular localisations.

Gian Gaetano Tartaglia1, Michele Vendruscolo

  • 1Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, UK.

Molecular Biosystems
|September 19, 2009
PubMed
Summary

Messenger RNA (mRNA) expression and protein aggregation show a strong correlation across cellular locations. Protein abundance and solubility increase in smaller cellular volumes, highlighting protein solubility's importance for protein homeostasis.

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Last Updated: Jun 20, 2026

Evaluation of the Impact of Protein Aggregation on Cellular Oxidative Stress in Yeast
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04:47

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Published on: May 22, 2020

Area of Science:

  • Proteomics
  • Molecular Biology
  • Cell Biology

Background:

  • Protein homeostasis is crucial for cellular function.
  • Understanding factors influencing protein solubility is key.
  • The relationship between mRNA expression and protein aggregation is not fully understood.

Purpose of the Study:

  • To investigate the correlation between mRNA expression levels and protein aggregation propensities.
  • To explore how subcellular localization volume affects these properties.
  • To elucidate the role of protein solubility in maintaining protein homeostasis.

Main Methods:

  • Proteomic analysis of mRNA expression levels.
  • Assessment of protein aggregation propensities.
  • Correlation analysis across subcellular localizations.
  • Investigation of the dependence on subcellular localization volume.

Main Results:

  • A significant correlation was observed between mRNA expression and protein aggregation when averaged across subcellular localizations.
  • Protein abundance and solubility increase as subcellular localization volumes decrease.
  • These findings suggest a link between cellular environment and protein properties.

Conclusions:

  • Protein solubility plays a critical role in maintaining protein homeostasis.
  • Subcellular localization volume is a significant factor influencing protein abundance and solubility.
  • The study provides new insights into the regulation of protein aggregation and cellular function.