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Related Concept Videos

Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
Overview of Transposition and Recombination02:13

Overview of Transposition and Recombination

Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
DNA-only Transposons02:57

DNA-only Transposons

DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
Transposons01:24

Transposons

Transposons, or "jumping genes," are small mobile genetic elements (MGEs) that range from 700 to 40,000 base pairs in length. They are found in all organisms and can move within the same chromosome or transfer to different chromosomes. In some cases, transposons can also jump between different host DNA molecules, such as plasmids or viruses, contributing to genetic variability.Barbara McClintock first discovered these mobile genetic elements in the 1940s while studying maize genetics, and she...

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Related Experiment Video

Updated: Jun 20, 2026

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

A c-Myc regulatory subnetwork from human transposable element sequences.

Jianrong Wang1, Nathan J Bowen, Leonardo Mariño-Ramírez

  • 1School of Biology, Georgia Institute of Technology, Atlanta, GA 30332, USA. jwang64@gatech.edu

Molecular Biosystems
|September 19, 2009
PubMed
Summary
This summary is machine-generated.

Transposable elements (TEs) contribute regulatory sequences to human gene expression. These TEs form distinct networks regulating genes, including those involved in cancer, through the oncogene c-Myc.

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Real-Time Quantification of the Effects of IS200/IS605 Family-Associated TnpB on Transposon Activity
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HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
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Real-Time Quantification of the Effects of IS200/IS605 Family-Associated TnpB on Transposon Activity
04:04

Real-Time Quantification of the Effects of IS200/IS605 Family-Associated TnpB on Transposon Activity

Published on: January 20, 2023

Area of Science:

  • Genomics
  • Molecular Biology
  • Cancer Research

Background:

  • Transposable elements (TEs) are mobile DNA sequences that can influence gene regulation.
  • The oncogene c-Myc is a transcription factor regulating numerous genes.
  • Understanding the interplay between TEs and c-Myc is crucial for deciphering gene regulation and cancer development.

Purpose of the Study:

  • To investigate the contribution of TEs to the c-Myc regulatory network.
  • To identify TE-derived c-Myc binding sites and their associated target genes.
  • To analyze the functional relevance of these TE-regulated genes in cancer.

Main Methods:

  • Genome-wide search for c-Myc binding sites within TEs.
  • Analysis of gene expression patterns for genes near TE-derived binding sites.
  • Comparison of identified TE-c-Myc targets with previously known c-Myc targets.
  • Differential gene expression analysis between cancer cells and normal cells.

Main Results:

  • Thousands of TE sequences in the human genome are bound by c-Myc.
  • A significant number of TEs contain canonical c-Myc binding motifs, particularly from ancient families L2 and MIR.
  • Genes associated with TE-derived c-Myc binding sites are co-expressed with c-Myc and show differential expression in Burkitt's lymphoma.
  • Identified TE-c-Myc targets represent a distinct regulatory subnetwork not significantly overlapping with previously known sets.

Conclusions:

  • TEs play a substantial role in regulating human genes via c-Myc binding.
  • TE-derived regulatory elements contribute to a distinct c-Myc regulatory subnetwork.
  • These findings highlight the importance of TEs in gene regulation and their potential role in cancer pathogenesis.