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Related Concept Videos

Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as  cells...
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
SDS-PAGE01:27

SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...

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Related Experiment Video

Updated: Jun 20, 2026

Light-Induced Dielectrophoresis for Characterizing the Electrical Behavior of Human Mesenchymal Stem Cells
10:08

Light-Induced Dielectrophoresis for Characterizing the Electrical Behavior of Human Mesenchymal Stem Cells

Published on: June 16, 2023

Dielectrophoresis as a cell characterisation tool.

Kai F Hoettges1

  • 1Centre for Biomedical Engineering, University of Surrey, Guilford, Surrey, UK.

Methods in Molecular Biology (Clifton, N.J.)
|September 19, 2009
PubMed
Summary

Dielectrophoresis (DEP) measures cell electrophysiology without labels by observing cell movement in electric fields. This chapter explores DEP theory, equipment, and practical considerations for its application.

Area of Science:

  • Biophysics
  • Cellular Electrophysiology
  • Biotechnology

Background:

  • Dielectrophoresis (DEP) is a label-free technique for measuring cell electrophysiology.
  • It relies on observing cell movement within non-uniform electric fields.
  • Advancements in instrumentation have improved DEP measurement capabilities.

Purpose of the Study:

  • To explore the fundamental theory underpinning dielectrophoresis.
  • To discuss the implications of new equipment for DEP measurements.
  • To address practical aspects of conducting DEP experiments, including medium selection.

Main Methods:

  • Theoretical exploration of dielectrophoresis principles.
  • Analysis of instrumentation development for DEP.
  • Discussion of experimental parameters, focusing on suspending media selection.

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Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis
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Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis

Published on: September 3, 2013

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
09:45

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

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Last Updated: Jun 20, 2026

Light-Induced Dielectrophoresis for Characterizing the Electrical Behavior of Human Mesenchymal Stem Cells
10:08

Light-Induced Dielectrophoresis for Characterizing the Electrical Behavior of Human Mesenchymal Stem Cells

Published on: June 16, 2023

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis
10:38

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis

Published on: September 3, 2013

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
09:45

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

Main Results:

  • Provides a comprehensive overview of DEP theory.
  • Highlights the impact of technological advancements on DEP applications.
  • Offers practical guidance for optimizing DEP experiments.

Conclusions:

  • Dielectrophoresis is a powerful tool for label-free cell electrophysiology.
  • Understanding DEP theory and equipment is crucial for accurate measurements.
  • Careful consideration of experimental conditions, such as the suspending medium, is essential for successful DEP applications.