Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

2D NMR: Overview of Heteronuclear Correlation Techniques01:18

2D NMR: Overview of Heteronuclear Correlation Techniques

Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other axis.
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
2D NMR: Homonuclear Correlation Spectroscopy (COSY)01:06

2D NMR: Homonuclear Correlation Spectroscopy (COSY)

Homonuclear correlation spectroscopy, or COSY, is a 2-dimensional NMR technique that provides information about coupled protons. Typically, the geminal and vicinal coupling are observed. For example, consider the COSY spectrum of ethyl acetate, where its 1D proton NMR spectrum is plotted along the vertical and horizontal axes with their corresponding chemical shift scale. Three spots on the diagonal corresponding to the three peaks in the 1D proton spectrum are called diagonal peaks. The COSY...
Two-Dimensional (2D) NMR: Overview01:12

Two-Dimensional (2D) NMR: Overview

The 1D NMR spectrum of large and complex molecules like natural products has complicated splitting patterns and overlapping signals, which can be easily interpreted using 2-dimensional (2D) NMR. Unlike 1D NMR, 2D NMR has two frequency axes that provide the coupling information between the nucleus A and nucleus B in a molecule. The process from which 2D spectra are obtained has four steps.
The first step is the preparation period, during which nucleus A is excited with a radiofrequency pulse.
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are slanted or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genus <i>Cucumis</i>: Traditional uses, phytochemistry, pharmacology, clinical application, and toxicology.

Chinese herbal medicines·2026
Same author

A virtual screening and molecular dynamics approach in search of novel antibiotic chemotypes.

PloS one·2026
Same author

Discovery of Sulfonamide Pantothenate Kinase Activators and Elucidation of the Role of Isoform Selectivity in Cellular Pantothenate Kinase Activation.

Journal of medicinal chemistry·2026
Same author

Amplicon sequencing of ice and water phytoplankton and bacterial communities during an extreme winter in a central Canadian great lake.

Microbiology resource announcements·2025
Same author

An open-source screening platform accelerates discovery of drug combinations.

Nature communications·2025
Same author

Mycobacterium tuberculosis overcomes phosphate starvation by extensively remodelling its lipidome with phosphorus-free lipids.

Nature communications·2025

Related Experiment Video

Updated: Jun 20, 2026

NMR Spectroscopy as a Robust Tool for the Rapid Evaluation of the Lipid Profile of Fish Oil Supplements
08:54

NMR Spectroscopy as a Robust Tool for the Rapid Evaluation of the Lipid Profile of Fish Oil Supplements

Published on: May 1, 2017

Lipid profiling using two-dimensional heteronuclear single quantum coherence NMR.

Engy A Mahrous1, Robin B Lee, Richard E Lee

  • 1Department of Pharmaceutical Sciences, College of Pharmacy, University of Tennessee Health Sciences Center, Memphis, TN, USA.

Methods in Molecular Biology (Clifton, N.J.)
|September 19, 2009
PubMed
Summary

Nuclear Magnetic Resonance (NMR) spectroscopy now enables comprehensive lipidome analysis, overcoming traditional limitations of sensitivity and signal overlap for complex biological samples like mycobacteria.

More Related Videos

A New Straightforward Method for Lipophilicity (logP) Measurement using 19F NMR Spectroscopy
09:32

A New Straightforward Method for Lipophilicity (logP) Measurement using 19F NMR Spectroscopy

Published on: January 30, 2019

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
09:38

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Published on: January 7, 2019

Related Experiment Videos

Last Updated: Jun 20, 2026

NMR Spectroscopy as a Robust Tool for the Rapid Evaluation of the Lipid Profile of Fish Oil Supplements
08:54

NMR Spectroscopy as a Robust Tool for the Rapid Evaluation of the Lipid Profile of Fish Oil Supplements

Published on: May 1, 2017

A New Straightforward Method for Lipophilicity (logP) Measurement using 19F NMR Spectroscopy
09:32

A New Straightforward Method for Lipophilicity (logP) Measurement using 19F NMR Spectroscopy

Published on: January 30, 2019

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
09:38

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Published on: January 7, 2019

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy

Background:

  • Nuclear Magnetic Resonance (NMR) spectroscopy traditionally used for discrete lipid molecule analysis.
  • Previous limitations in global lipid pool analysis due to low sensitivity and signal overlap.
  • Recent advancements have significantly improved NMR sensitivity and sampling flexibility.

Purpose of the Study:

  • To describe a methodology for global lipidome analysis using NMR spectroscopy.
  • To leverage NMR's quantitative nature and structural information for lipidomics.
  • To address challenges of low sensitivity and signal overlap in complex lipid mixtures.

Main Methods:

  • Utilized isotope-enrichment to enhance sensitivity.
  • Employed multidimensional NMR to resolve overlapping signals.
  • Applied the methodology to the complex mycobacterial lipidome.

Main Results:

  • Successfully performed global lipidome analysis on a complex biological sample.
  • Demonstrated the quantitative and structural information-rich capabilities of NMR for lipidomics.
  • Overcame traditional sensitivity and signal resolution limitations.

Conclusions:

  • NMR spectroscopy is a powerful tool for global lipidome analysis.
  • The described method is effective for complex lipid pools, exemplified by mycobacteria.
  • This approach is applicable to a broad range of prokaryotes grown in defined media.