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Related Concept Videos

Oogenesis02:07

Oogenesis

In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
What is Gene Expression?01:36

What is Gene Expression?

A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then processed and...

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Cell-Specific Paired Interrogation of the Mouse Ovarian Epigenome and Transcriptome
12:25

Cell-Specific Paired Interrogation of the Mouse Ovarian Epigenome and Transcriptome

Published on: February 24, 2023

Gene expression profiling in the aging ovary.

Kathleen M Eyster1, John D Brannian

  • 1Division of Basic Biomedical Sciences, Sanford School of Medicine, University of South Dakota, Vermillion, SD, USA.

Methods in Molecular Biology (Clifton, N.J.)
|September 19, 2009
PubMed
Summary
This summary is machine-generated.

DNA microarrays enable simultaneous analysis of thousands of genes, revealing novel gene expression patterns. This technology aids in understanding complex biological processes in hormone-responsive tissues like the ovary.

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Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • DNA microarrays are a powerful tool for high-throughput gene expression analysis.
  • Understanding gene expression is crucial for elucidating biological processes and disease mechanisms.

Purpose of the Study:

  • To highlight the utility of DNA microarray technology.
  • To demonstrate its application in studying gene expression in hormone-responsive tissues.

Main Methods:

  • Utilizes DNA microarray technology for large-scale gene expression profiling.
  • Analyzes differential gene expression patterns.

Main Results:

  • Identifies genes with altered expression not previously associated with specific biological processes.
  • Provides insights into the effects of various factors on gene expression.

Conclusions:

  • DNA microarrays are essential for discovering novel gene functions and understanding complex biological systems.
  • The technology is valuable for research in endocrinology, aging, and disease, particularly in the ovary.