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Related Concept Videos

Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.

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Related Experiment Video

Updated: Jun 20, 2026

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin
24:02

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin

Published on: April 11, 2014

Chromosome-wide analysis of protein binding and modifications.

Kevin D Sarge1, Hongyan Xing, Ok-Kyong Park-Sarge

  • 1Department of Molecular and Cellular Biochemistry, University of Kentucky College of Medicine, Lexington, KY, USA.

Methods in Molecular Biology (Clifton, N.J.)
|September 19, 2009
PubMed
Summary

Identifying DNA-binding protein locations on chromosomes is crucial for understanding their function. ChIP-on-Chip technology enables chromosome-wide mapping of these sites and protein modifications, exemplified by TATA-binding protein analysis.

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Last Updated: Jun 20, 2026

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin
24:02

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Published on: December 28, 2012

Area of Science:

  • Molecular Biology
  • Genomics
  • Epigenetics

Background:

  • Understanding DNA-binding protein functions requires identifying all chromosomal binding sites.
  • Assessing the role of each binding site is essential for comprehending protein functions.

Purpose of the Study:

  • To present methodologies for Chromatin Immunoprecipitation-on-Chip (ChIP-on-Chip) analysis.
  • To demonstrate the identification of chromosome-wide binding sites for a specific protein.

Main Methods:

  • ChIP-on-Chip combines chromatin immunoprecipitation with DNA microarray analysis.
  • This technique allows for the identification of protein binding sites across entire chromosomes.
  • It can also characterize chromosome-wide variations in post-translational protein modifications, such as histone modifications.

Main Results:

  • ChIP-on-Chip is a powerful approach for identifying protein binding sites genome-wide.
  • The chapter provides a practical example using TATA-binding protein in mitotic cells.
  • Methodologies for analyzing chromosome-wide binding patterns are detailed.

Conclusions:

  • ChIP-on-Chip is an effective method for mapping protein-DNA interactions genome-wide.
  • This approach is valuable for studying protein functions and epigenetic variations.
  • The presented methodologies facilitate the comprehensive analysis of DNA-binding proteins.