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Updated: Jun 20, 2026

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Athymic Rat Model for Evaluation of Engineered Anterior Cruciate Ligament Grafts
Published on: March 26, 2015
Biomechanical evaluation of using one hamstrings tendon for ACL reconstruction: a human cadaveric study
Giovanni Zamarra1, Matthew B Fisher, Savio L-Y Woo
1Department of Bioengineering, Swanson School of Engineering, University of Pittsburgh, Musculoskeletal Research Center, 405 Center for Bioengineering, 300 Technology Drive, Pittsburgh, PA 15219, USA.
Summary
The "All-inside" technique for anterior cruciate ligament (ACL) reconstruction using a single hamstring tendon graft effectively restores knee function and stability, matching intact knee performance.
Area of Science:
- Orthopedic Surgery
- Biomechanical Engineering
- Sports Medicine
Background:
- Harvesting both semitendinosus and gracilis tendons for ACL reconstruction can negatively impact muscle strength and knee function.
- The
- All-inside
- ACL reconstruction technique allows for the use of a single hamstring tendon graft due to reduced length requirements.
Purpose of the Study:
- To evaluate if a single semitendinosus or gracilis tendon graft can restore knee kinematics and ACL in situ force to the level of an intact knee.
- To compare the biomechanical outcomes of the
- All-inside
- ACL reconstruction technique using single hamstring tendon grafts against an intact ACL.
Main Methods:
- Ten human cadaveric knees were tested under intact, ACL-deficient, and ACL reconstruction conditions.
- ACL reconstruction was performed using the
- All-inside
- technique with either a single semitendinosus or gracilis tendon graft.
- A robotic testing system applied anterior tibial loads and combined rotatory loads to assess knee kinematics and in situ forces.
Main Results:
- Both single semitendinosus and gracilis tendon grafts restored anterior tibial translation to within 1.3 mm of the intact knee under anterior tibial load.
- In situ forces in both grafts were comparable to the intact ACL.
- Under combined rotatory loads, both grafts successfully restored knee kinematics and in situ forces to the levels of an intact ACL.
Conclusions:
- The
- All-inside
- ACL reconstruction technique using a single semitendinosus or gracilis tendon graft satisfactorily restores time-zero knee kinematics.
- This technique effectively restores in situ forces in the graft to match those of an intact ACL.
- The findings support the clinical efficacy of the
- All-inside
- technique for ACL reconstruction.
