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Related Experiment Video

Updated: Jun 20, 2026

A High-throughput Method for Measurement of Glomerular Filtration Rate in Conscious Mice
07:07

A High-throughput Method for Measurement of Glomerular Filtration Rate in Conscious Mice

Published on: May 10, 2013

Measuring kidney function in conscious mice.

David L Mattson1

  • 1Department of Physiology, Medical College of Wisconsin, Milwaukee, WI, USA.

Methods in Molecular Biology (Clifton, N.J.)
|September 19, 2009
PubMed
Summary
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Researchers developed methods to measure kidney function in conscious mice using surgical catheters. This allows for accurate physiological assessments in freely moving animals, advancing renal research.

Area of Science:

  • Physiology
  • Nephrology
  • Animal Models

Background:

  • Mouse models are crucial for studying kidney function and homeostasis.
  • Technical limitations hinder the full use of mouse models in renal research.
  • Current methods often do not reflect physiological conditions in freely moving animals.

Purpose of the Study:

  • To present methods for quantifying renal function in conscious, freely moving mice.
  • To overcome technical limitations in current mouse models for renal research.
  • To enable in vivo assessment of kidney physiology in a natural state.

Main Methods:

  • Surgical instrumentation of mice with chronic indwelling arterial and venous catheters.
  • Direct measurement of arterial blood pressure and blood sampling.

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Transcutaneous Assessment of Renal Function in Conscious Rodents
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Transcutaneous Assessment of Renal Function in Conscious Rodents

Published on: March 26, 2016

Assessment of Kidney Function in Mouse Models of Glomerular Disease
09:16

Assessment of Kidney Function in Mouse Models of Glomerular Disease

Published on: June 30, 2018

Related Experiment Videos

Last Updated: Jun 20, 2026

A High-throughput Method for Measurement of Glomerular Filtration Rate in Conscious Mice
07:07

A High-throughput Method for Measurement of Glomerular Filtration Rate in Conscious Mice

Published on: May 10, 2013

Transcutaneous Assessment of Renal Function in Conscious Rodents
07:18

Transcutaneous Assessment of Renal Function in Conscious Rodents

Published on: March 26, 2016

Assessment of Kidney Function in Mouse Models of Glomerular Disease
09:16

Assessment of Kidney Function in Mouse Models of Glomerular Disease

Published on: June 30, 2018

  • Intravenous or intra-arterial infusion of substances and quantification of sodium balance.

    • Main Results:

    • Developed a preparation for measuring renal function in conscious mice.
    • Enabled direct physiological measurements in freely moving animals.
    • Facilitated assessment of kidney function in a native physiological state.

    Conclusions:

    • The described techniques allow for comprehensive physiological assessment in conscious mice.
    • These methods overcome limitations of traditional mouse models in renal research.
    • This in vivo preparation provides a more accurate reflection of kidney function.