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Related Experiment Video

Updated: Jun 20, 2026

Evaluating Cell Death Signaling by Immunofluorescence in a Rat Model of Ischemic Stroke
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Evaluating Cell Death Signaling by Immunofluorescence in a Rat Model of Ischemic Stroke

Published on: January 3, 2025

Ischemic stroke in mice and rats.

Aysan Durukan1, Turgut Tatlisumak

  • 1Department of Neurology, Helsinki University Central Hospital, Helsinki, Finland.

Methods in Molecular Biology (Clifton, N.J.)
|September 19, 2009
PubMed
Summary
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The middle cerebral artery (MCA) occlusion model in rodents is a reliable method for studying ischemic stroke. This technique allows for controlled induction of ischemia and reperfusion, aiding neuroprotective drug development.

Area of Science:

  • Neuroscience
  • Cerebrovascular Research
  • Ischemic Stroke Models

Background:

  • Ischemic stroke frequently affects the middle cerebral artery (MCA) territory in humans.
  • The intraluminal suture occlusion of the MCA in rodents is a widely adopted stroke model.
  • This model offers controlled induction of ischemia and reperfusion, crucial for research.

Purpose of the Study:

  • To detail the application and benefits of the intraluminal MCA occlusion model in rodents.
  • To highlight its utility in evaluating neuroprotective agents for ischemic stroke.
  • To describe outcome measures for assessing treatment efficacy.

Main Methods:

  • Minimally invasive intraluminal suture occlusion of the MCA in rats and mice.
  • Guidance using laser-Doppler flowmetry for precise occlusion/reperfusion control.

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Focal Cerebral Ischemia Model by Endovascular Suture Occlusion of the Middle Cerebral Artery in the Rat
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Focal Cerebral Ischemia Model by Endovascular Suture Occlusion of the Middle Cerebral Artery in the Rat

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Induction of Ischemic Stroke and Ischemia-reperfusion in Mice Using the Middle Artery Occlusion Technique and Visualization of Infarct Area
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Induction of Ischemic Stroke and Ischemia-reperfusion in Mice Using the Middle Artery Occlusion Technique and Visualization of Infarct Area

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Last Updated: Jun 20, 2026

Evaluating Cell Death Signaling by Immunofluorescence in a Rat Model of Ischemic Stroke
11:32

Evaluating Cell Death Signaling by Immunofluorescence in a Rat Model of Ischemic Stroke

Published on: January 3, 2025

Focal Cerebral Ischemia Model by Endovascular Suture Occlusion of the Middle Cerebral Artery in the Rat
13:50

Focal Cerebral Ischemia Model by Endovascular Suture Occlusion of the Middle Cerebral Artery in the Rat

Published on: February 5, 2011

Induction of Ischemic Stroke and Ischemia-reperfusion in Mice Using the Middle Artery Occlusion Technique and Visualization of Infarct Area
09:08

Induction of Ischemic Stroke and Ischemia-reperfusion in Mice Using the Middle Artery Occlusion Technique and Visualization of Infarct Area

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  • Induction of 90 minutes of transient focal cerebral ischemia.

    • Main Results:

    • The MCA occlusion model reliably induces ischemic lesions in the striatum and cortex.
    • Lesions exhibit characteristics of both ischemic core and penumbra.
    • Reproducible outcomes facilitate lesion and neurological deficit assessment.

    Conclusions:

    • The intraluminal MCA occlusion model is highly suitable for preclinical studies of neuroprotective drugs.
    • It enables robust evaluation of ischemic brain injury and neurological function.
    • This model supports the development of effective stroke therapies.