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Related Experiment Video

Updated: Jun 20, 2026

High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy
15:13

High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy

Published on: July 25, 2014

Imaging B-cell receptor signaling by single-molecule techniques.

Pavel Tolar1, Tobias Meckel

  • 1Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, NIH, Rockville, MD, USA.

Methods in Molecular Biology (Clifton, N.J.)
|September 19, 2009
PubMed
Summary

New single-molecule imaging techniques reveal the initial molecular steps of B-cell antigen receptor (BCR) microclustering and activation. These methods track BCR behavior during dynamic cell-cell interactions, advancing our understanding of immune responses.

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Published on: March 22, 2012

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Last Updated: Jun 20, 2026

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Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell
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A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
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A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

Published on: March 22, 2012

Area of Science:

  • Immunology
  • Cell Biology
  • Biophysics

Background:

  • B-cell activation is crucial for antibody production against pathogens.
  • B cells rapidly scan lymphoid tissues for antigens presented by antigen-presenting cells.
  • Antigen recognition by the B-cell antigen receptor (BCR) triggers signaling, B-cell arrest, and synapse formation.

Purpose of the Study:

  • To review single-molecule imaging techniques for studying early BCR activation events.
  • To elucidate the elusive initial molecular steps of BCR microclustering and signaling.
  • To provide insights into the behavior of BCRs during dynamic cell-cell interactions.

Main Methods:

  • Review of single-molecule imaging techniques.
  • Analysis of BCR dynamics in live B cells during antigen encounter.
  • Observation of microclustering and initial signaling events at the molecular level.

Main Results:

  • Single-molecule imaging provides unprecedented views of BCR behavior.
  • These techniques overcome challenges posed by dynamic cell-cell contacts.
  • Molecular dynamics of BCR microclustering and initiation of signaling are illuminated.

Conclusions:

  • Single-molecule imaging is essential for understanding early BCR activation.
  • These techniques offer a powerful approach to studying membrane receptor dynamics.
  • The findings advance our knowledge of B-cell immune responses and cell-cell communication.