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Updated: Jun 20, 2026

Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade
Published on: August 14, 2019
[HPLC fingerprint of flavonoids and phenols of Dendrobium nobile]
Huan-Jiao Ou1, Jin-Le Cheng, Xian-Xia Li
1Guangzhou University of Chinese Medicine, Guangzhou 510006, China. ouhuanjiao@163.com
Objective:
To establish HPLC fingerprint of flavonoids and phenols of Dendrobium nobile.
Methods:
Phenomenex prodigy ODS(3) C18 column (250 mm x 4.6 mm, 5 microm) was used with a mixture of acetonitrile-0.1% acetic acid as the mobile phase in a gradient mode, the column temperature was 25 degrees C, the flow rate was 1.0 mL/min, and the detection wavelength was 254 nm.
Results:
The flavonoids and phenols of Dendrobium nobile were well separated, and 10 fingerprint peaks in common were confirmed.
Conclusion:
This method is simple, accurate with good reproducibility, and can be used specifically for the quality control of Dendrobium nobile.
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