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[Simultaneous determination of anthraquinones in Liuwei'anxiao capsules by HPLC]
Bo Jiang1, Xun Gao, Kai-Shun Bi
1School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China. rabbitbo@sina.com
Objective:
To establish an RP-HPLC method for measuring the contents of aloe-emodin, rhein, emodin, chrysophanol and physion in Liuwei'anxiao Capsules (traditional Chinese medicine).
Methods:
The analysis was carried out with a Diamonsil-C18 column (200 mm x 4.6 mm, 5 microm) and a mobile phase of methanol-0.05% phosphoric acid solution with gradient elution. The flow rate was 1.0 mL/min and the column temperature was 35 degrees C. The detection wavelength was 254 nm.
Results:
The calibration curve was linear within the range of 1.622 - 16.22 microg/mL (r1 = 0.9998), 0.6960 - 6.960 microg/mL (r2 = 0.9998), 2.448 - 24.48 microg/mL (r3 = 0.9995), 2.752 - 27.52 microg/mL (r4 = 0.9997), 2.702 -27.02 microg/mL (r = 0.9997) for aloe-emodin, rhein, emodin, chrysophanol and physion, respectively.
Conclusion:
This method is convenient, accurate and reproducible for the quality control of Liuwei'anxiao Capsules.
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