Related Experiment Video
Updated: Jun 20, 2026
Induction of Drug-Induced, Autoimmune Hepatitis in BALB/c Mice for the Study of Its Pathogenic Mechanisms
Published on: May 29, 2020
[SKLB-102 inhibits acute hepatitis induced by concanavalin A in mice]
Chun-Mei He1, Lu-Lu Cai, Hao Zheng
1State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China.
Objective:
To test the effect of 5-(4-methoxybenzylidene)-2-thioxo-dihydropyrimidine-4, 6 (1H, 5H)-dione (SKLB-102) on acute hepatic inflammatory induced by concanavalin A (ConA) in mice.
Methods:
The inhibitive effect of SKLB-102 on RAW264.7 cell migration induced by recombinant rat monocyte chemotactic protein-1 (MCP-1) was tested. The serum from the ConA-treated mice was collected after intragastric administration of SKLB-102 at the dose of 50 mg/kg bodyweight. The serum AST and ALT were determined by an automatic analyzer, and the serum TNF-alpha was determined with enzyme-linked immunosorbent assay (ELISA) kits. The liver samples were fixed in 10% formalin, embedded in paraffin, sectioned and then stained with hematoxylin and eosin for histological examinations.
Results:
SKLB-102 markedly reduced cell migrations, successfully reduced serum AST, ALT and down-regulated TNF-alpha.
Conclusion:
SKLB-102 is likely to suppress the occurrence of Con A-induced hepatitis by suppressing macrophages migration and TNF-alpha releases.
