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Potentiation of Anticancer Antibody Efficacy by Antineoplastic Drugs: Detection of Antibody-drug Synergism Using the Combination Index Equation
Published on: January 19, 2019
Polymer-drug conjugates for combination anticancer therapy: investigating the mechanism of action
Gianfranco Pasut1, Francesca Greco, Anna Mero
1Department of Pharmaceutical Sciences, Via F. Marzolo 5, University of Padua, 35100 Padova, Italy. gianfranco.pasut@unipd.it
Abstract:
We developed a family of polymer-drug conjugates carrying the combination of the anticancer agent epirubicin (EPI) and nitric oxide (NO). EPI-PEG-(NO)8, carrying the highest content of NO, displayed greater activity in Caco-2 cells while it decreased toxicity against endothelium cells and cardiomyocytes with respect to free EPI. FACS and confocal microscopy confirmed conjugates internalization. Light scattering showed formation of micelle whose size correlated with internalization rate. EPI-PEG-(NO)8 showed increased bioavailability in mice compared to free EPI.
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