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Related Concept Videos

RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

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RNA interference in schistosomes: machinery and methodology.

Greice Krautz-Peterson1, Rita Bhardwaj, Zahra Faghiri

  • 1Molecular Helminthology Laboratory, Division of Infectious Diseases, Department of Biomedical Sciences, Tufts University, Cummings School of Veterinary Medicine, Grafton, MA 01536, USA.

Parasitology
|September 22, 2009
PubMed
Summary

RNA interference (RNAi) effectively silences genes in schistosomes. New methods show robust gene suppression using short interfering RNAs (siRNAs) delivered via soaking or electroporation, even in mixed-gender pairs.

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Area of Science:

  • Parasitology
  • Molecular Biology
  • Genetics

Background:

  • RNA interference (RNAi) is crucial for studying gene function in schistosomes.
  • Understanding RNAi methodology and machinery in these parasites is essential.

Purpose of the Study:

  • To review and update knowledge on RNAi processes in schistosomes.
  • To present new data on RNAi efficiency and machinery in schistosome parasites.
  • To identify potential RNAi pathways in schistosomes.

Main Methods:

  • Investigating gene suppression levels using various RNAi delivery methods.
  • Analyzing the efficiency of short interfering RNAs (siRNAs) via soaking and electroporation.
  • In silico identification of RNAi machinery components, including SID-1 homologues.

Main Results:

  • Not all schistosome genes are equally suppressible by current RNAi methods.
  • Robust (>80%) suppression of the SmAP gene was achieved in adult schistosomes.
  • siRNAs effectively suppress genes when delivered by soaking or electroporation, with no need to separate male/female pairs.
  • Electroporation in medium is as effective as commercial buffer for siRNA delivery.
  • A schistosome homologue of the RNA importing protein SID-1 (SmSID-1) was identified.

Conclusions:

  • RNAi is a versatile tool for schistosome gene function studies.
  • Optimized methods enhance siRNA delivery and gene suppression efficiency.
  • Identification of SmSID-1 provides insights into schistosome RNAi pathway mechanisms.