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Related Concept Videos

Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
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Production of Nanofibrillar Patterned Collagen for Tissue Engineering
07:34

Production of Nanofibrillar Patterned Collagen for Tissue Engineering

Published on: September 20, 2024

Dynamic shear-influenced collagen self-assembly.

Nima Saeidi1, Edward A Sander, Jeffrey W Ruberti

  • 1Department of Mechanical and Industrial Engineering, Northeastern University, 334 Snell Engineering, Boston, MA 02115, USA. saeidi@coe.neu.edu

Biomaterials
|September 22, 2009
PubMed
Summary
This summary is machine-generated.

Flow influences collagen self-assembly, but achieving native-like, aligned fibrils is challenging. Optimizing shear and surface properties may improve biomaterial development for tissue engineering.

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Microengineering 3D Collagen Hydrogels with Long-Range Fiber Alignment
07:12

Microengineering 3D Collagen Hydrogels with Long-Range Fiber Alignment

Published on: September 7, 2022

Area of Science:

  • Biomolecular self-assembly
  • Materials science
  • Tissue engineering

Background:

  • Highly anisotropic materials can be generated by controlling biomolecular self-assembly direction.
  • Aligned molecular scaffolds are crucial for guiding cells in engineered tissues like cornea and tendon.

Purpose of the Study:

  • To investigate the dynamics of collagen type I assembly on glass surfaces under varying shear rates.
  • To analyze the morphology and alignment of collagen fibrils formed during flow-induced self-assembly.

Main Methods:

  • Differential Interference Contrast (DIC) imaging to track collagen aggregate growth at different shear rates (9-500 s⁻¹).
  • Quick Freeze Deep Etch (QFDE) electron microscopy to examine fibril morphology.
  • Controlled flow of pepsin-extracted type I bovine collagen between two plates over a glass surface.

Main Results:

  • Rapid collagen fibril nucleation (approx. 2 min) followed by continued growth.
  • Axial growth rates were complexly dependent on shear, with peak growth at 9 s⁻¹ and lowest at highest shear.
  • Best fibril alignment occurred at intermediate shear rates (20-80 s⁻¹), but growth was unstable, forming 'hooks' at high shear.
  • QFDE revealed fibrils lacked native D-periodicity, appearing as aligned monomers.

Conclusions:

  • Constant shear rate influences collagen fibril alignment but does not produce native-like, D-banded structures.
  • Achieving highly organized, native-like collagenous arrays remains a challenge.
  • Combining modulated shear with surface energy patterning could enhance fibril morphology and alignment for tissue engineering applications.