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Updated: Jun 20, 2026

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
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Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

Surface plasmon resonance imaging for affinity-based biosensors.

Simona Scarano1, Marco Mascini, Anthony P F Turner

  • 1Dipartimento di Chimica, Università degli Studi di Firenze-Polo Scientifico, Sesto F.no (FI), Italy.

Biosensors & Bioelectronics
|September 22, 2009
PubMed
Summary
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Surface Plasmon Resonance imaging (SPRi) enables label-free, real-time monitoring of hundreds of biological interactions. This review covers SPRi technology, applications, and methods to enhance sensitivity for kinetic analysis.

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Biophysics

Background:

  • Surface Plasmon Resonance imaging (SPRi) is a key optical technique for label-free, real-time biomolecular interaction analysis.
  • SPRi allows simultaneous monitoring of numerous interactions, providing insights into binding kinetics.

Purpose of the Study:

  • To review the current development and applications of SPRi technology.
  • To discuss commercially available SPRi instruments.
  • To explore surface chemistries and methods for enhancing SPRi sensitivity.

Main Methods:

  • Review of existing literature and commercial SPRi instrumentation.
  • Analysis of surface functionalization strategies for biochip development.
  • Evaluation of techniques for improving detection sensitivity.

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Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)
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Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)

Published on: November 29, 2014

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Last Updated: Jun 20, 2026

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
06:12

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)
09:35

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)

Published on: November 29, 2014

Main Results:

  • SPRi technology is advancing rapidly, offering high-throughput analysis of biological interactions.
  • Commercial SPRi instruments are available, facilitating diverse research applications.
  • Optimized surface chemistries and novel approaches can significantly improve SPRi sensitivity.

Conclusions:

  • SPRi is a powerful tool for label-free, real-time kinetic analysis of biomolecular interactions.
  • Continued development in surface chemistry and instrumentation promises further advancements in SPRi sensitivity and application scope.