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Related Concept Videos

X-ray Crystallography02:18

X-ray Crystallography

The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...

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Updated: Jun 20, 2026

High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography
06:19

High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

Published on: March 10, 2023

High-throughput crystallography for structural genomics.

Andrzej Joachimiak1

  • 1Midwest Center for Structural Genomics, Structural Biology Center, Biosciences Division, Argonne National Laboratory, 9700 S Class Ave., Argonne, IL 60439, USA. andrzejj@anl.gov

Current Opinion in Structural Biology
|September 22, 2009
PubMed
Summary

Structural genomics and high-throughput X-ray crystallography have revolutionized protein structure determination. Advances in technology and automation have accelerated progress in structural molecular biology.

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Protein Crystallization for X-ray Crystallography
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Protein Crystallization for X-ray Crystallography

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Last Updated: Jun 20, 2026

High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography
06:19

High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

Published on: March 10, 2023

Workflow and Tools for Crystallographic Fragment Screening at the Helmholtz-Zentrum Berlin
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Workflow and Tools for Crystallographic Fragment Screening at the Helmholtz-Zentrum Berlin

Published on: March 3, 2021

Protein Crystallization for X-ray Crystallography
09:27

Protein Crystallization for X-ray Crystallography

Published on: January 16, 2011

Area of Science:

  • Structural Molecular Biology
  • Biophysics
  • Biochemistry

Background:

  • Protein X-ray crystallography has a 50-year history, with early milestones including myoglobin and hemoglobin structures.
  • Over 55,000 protein structures are now in the Protein Data Bank, reflecting significant field advancement.

Purpose of the Study:

  • To review structural genomics high-throughput X-ray crystallography technologies.
  • To assess the impact of these technologies on the field of structural molecular biology.

Main Methods:

  • Review of advancements in macromolecular crystallography driven by structural genomics.
  • Integration of third-generation synchrotron sources, anomalous signal phasing, and cryo-crystallography.
  • Synergistic progress in molecular biology, proteomics, and automation technologies.

Main Results:

  • High-throughput X-ray crystallography technologies have been significantly advanced by structural genomics efforts.
  • Progress in synchrotron sources, phasing methods, and cryo-crystallography has been crucial.
  • Improvements in data collection, structure determination, refinement, and automation have accelerated processes.

Conclusions:

  • Advancements in X-ray crystallography provide a robust foundation for structural molecular biology.
  • Structural genomics and associated technologies ensure continued strong contributions to scientific discovery.
  • The impact of high-throughput technologies is transformative for understanding protein architecture and chemistry.