Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying DNA...
Epigenetic Regulation01:37

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
Histone Modification02:32

Histone Modification

The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Duplication of Chromatin Structure02:05

Duplication of Chromatin Structure

The process of chromosome duplication during cell division requires genome-wide disruption and re-assembly of chromatin. The chromatin structure must be accurately inherited, reassembled, and maintained in the daughter cells to ensure lineage propagation.
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Seroprevalence of Toxoplasma gondii and Feline Immunodeficiency Virus in Domestic Cats and Their Associations with Clinical Signs.

Acta parasitologica·2026
Same author

Molecular detection of Anaplasma phagocytophilum, Borrelia burgdorferi s.l., Coxiella burnetii, Ehrlichia chaffeensis and Rickettsia spp. in ticks collected from wild animals in six provinces of South Africa.

Acta tropica·2026
Same author

European Biophysics Journal - Special Issue Celebrating the 70th Anniversary of the Institute of Biophysics, Czech Academy of Sciences.

European biophysics journal : EBJ·2025
Same author

Biophysics in the Czech Republic: 70th anniversary of the institute of biophysics.

European biophysics journal : EBJ·2025
Same author

Specific TP53 mutations impair the recruitment of 53BP1 to DNA double-strand breaks underlying the mechanism of radioresistance.

European biophysics journal : EBJ·2025
Same author

Molecular Detection of <i>Borrelia burgdorferi</i> s.l. (<i>Borreliella</i>) and <i>Chlamydia</i>-Like Organism DNA in Early Developmental Stages of Arthropod Vector Species.

Transboundary and emerging diseases·2025

Related Experiment Video

Updated: Jun 20, 2026

Capturing Common Fragile Site Breaks by Native &#947;H2A.X ChIP
09:46

Capturing Common Fragile Site Breaks by Native γH2A.X ChIP

Published on: January 24, 2025

Chromocentre integrity and epigenetic marks.

Andrea Harnicarová Horáková1, Gabriela Galiová, Sona Legartová

  • 1Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i., Královopolská 135, CZ-612 65 Brno, Czech Republic.

Journal of Structural Biology
|September 22, 2009
PubMed
Summary

Epigenetic marks like histone methylation and acetylation influence how centromeres are organized within the nucleus. SUV39h deficiency and hyperacetylation repositioned these structures, revealing interchangeable roles for epigenetic marks in maintaining genome stability.

More Related Videos

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues
10:41

An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues

Published on: April 5, 2018

Related Experiment Videos

Last Updated: Jun 20, 2026

Capturing Common Fragile Site Breaks by Native &#947;H2A.X ChIP
09:46

Capturing Common Fragile Site Breaks by Native γH2A.X ChIP

Published on: January 24, 2025

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues
10:41

An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues

Published on: April 5, 2018

Area of Science:

  • Epigenetics and Molecular Biology
  • Genomics and Chromosome Biology
  • Cellular and Nuclear Organization

Background:

  • Histone modifications regulate chromatin structure, defining euchromatin and heterochromatin.
  • Centromeric heterochromatin forms distinct nuclear structures called chromocentres.
  • SUV39h is a key histone methyltransferase involved in heterochromatin formation.

Purpose of the Study:

  • To investigate the impact of SUV39h deficiency and trichostatin A (TSA)-induced hyperacetylation on chromocentre structural stability.
  • To determine how these epigenetic changes affect the nuclear positioning of chromocentres.
  • To elucidate the roles of specific epigenetic marks in maintaining centromeric heterochromatin integrity.

Main Methods:

  • Analysis of chromocentre structure and nuclear positioning in mouse cells with altered SUV39h levels and/or histone acetylation.
  • Assessment of key epigenetic marks including H3K9 methylation (H3K9me1, H3K9me2, H3K9me3), DNA methylation, and HP1 protein levels.
  • Comparative analysis of epigenetic modifications in SUV39h deficient versus wild-type cells.

Main Results:

  • Neither SUV39h deficiency nor hyperacetylation caused chromocentre disintegration as expected.
  • Both SUV39h deficiency and hyperacetylation in wild-type cells led to chromocentres moving towards the nuclear periphery.
  • TSA treatment in SUV39h-deficient cells restored normal chromocentre radial positioning.
  • Reciprocal changes in H3K9me1, H3K9me3, DNA methylation, and HP1 influenced chromocentre composition and arrangement.
  • H3K9me1 appeared to functionally substitute for H3K9me3 in chromocentre organization.

Conclusions:

  • Epigenetic modifications play crucial, interchangeable roles in maintaining chromocentre integrity and nuclear organization.
  • A model for the epigenetic regulation of nuclear stability in mouse centromeric heterochromatin is proposed.
  • Specific histone marks and their interplay are vital for the structural integrity and positioning of centromeric heterochromatin.