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The completion of the Mammalian Gene Collection (MGC)
Genome Research
|September 22, 2009
Summary
The Mammalian Gene Collection (MGC) now offers full-protein-coding sequence cDNA clones for most human and mouse genes. This resource, crucial for genetic research, provides access to over 6300 rat genes as well.
Area of Science:
- Genomics
- Molecular Biology
- Biotechnology
Background:
- The Mammalian Gene Collection (MGC) aims to provide comprehensive cDNA clones for mammalian genes.
- Initial MGC efforts relied on random expressed sequence tag screening.
Purpose of the Study:
- To report progress on MGC cloning using directed RT-PCR and DNA synthesis.
- To update the MGC clone collection status for human, mouse, and rat genes.
Main Methods:
- Utilized directed reverse transcription-polymerase chain reaction (RT-PCR) cloning.
- Employed DNA synthesis techniques for clone generation.
- Leveraged curated RefSeq transcripts and PubMed publications for gene selection.
Main Results:
- MGC now contains full-protein-coding sequence cDNA clones for 92% of human and 89% of mouse genes with RefSeq transcripts.
- Coverage increases to 97% for human and 96% for mouse genes with associated PubMed publications.
- Over 6300 rat genes are included in the MGC collection.
Conclusions:
- The MGC has significantly advanced its goal of providing comprehensive mammalian gene clones.
- High-quality MGC clones and sequences are freely accessible to the global research community.
- These resources facilitate mammalian gene research and functional studies.
