Real-time fluorescence detection of exoribonucleases
Flore Sinturel1, Olivier Pellegrini, Song Xiang
1Institut de Biologie Physico-Chimique, CNRS UPR 9073, Université de Paris 7-Denis Diderot, 75005 Paris, France.
RNA (New York, N.Y.)
|September 22, 2009
Abstract
None:
The identification of RNases or RNase effectors is a continuous challenge, particularly given the current importance of RNAs in the control of genome expression. Here, we show that a fluorogenic RNA-DNA hybrid is a powerful tool for a real-time fluorescence detection and assay of exoribonucleases (RT-FeDEx). This RT-FeDEx assay provides a new strategy for the isolation, purification, and assay of known and unknown exoribonucleases.
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