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Published on: December 21, 2010
Involvement of RecG in H2O2-induced damage repair in Deinococcus radiodurans
Yuanyuan Wu1, Weiwei Chen, Ye Zhao
1Key Laboratory for Nuclear-Agricultural Sciences of Chinese Ministry of Agriculture and Zhejiang Province, Institute of Nuclear-Agricultural Sciences, Zhejiang University, 310029 Hangzhou, China.
Abstract:
Deinococcus radiodurans (ex Raj et al. 1960) Brooks and Murray 1981 is well known for its efficient repair of various types of physically or chemically induced DNA damage caused by ionizing and ultraviolet radiation or H2O2. RecG codes for a helicase that is known to be involved in repairing oxidative damage in other bacterium. In this work, we constructed a DRrecG deletion mutant and investigated its possible role in H2O2-induced damage. The results showed that the deletion of DRrecG resulted in an obvious growth defect and great decrease of radioresistance of D. radiodurans to gamma radiation and H2O2. We also defined the transcriptional profiles of the recG mutant and wild-type strain with and without treatment with H2O2. These results suggested that DRrecG is important for DNA repair during oxidative damage.
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