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An Automated Method to Perform The In Vitro Micronucleus Assay using Multispectral Imaging Flow Cytometry
Published on: May 13, 2019
Cytology by Infrared Micro-Spectroscopy: Automatic Distinction of Cell Types in Urinary Cytology
Benjamin Bird1, Melissa J Romeo, Max Diem
1Department of Chemistry and Chemical Biology, Northeastern University, Hurtig Hall, Boston, MA 02115.
Abstract:
We report microscopically collected infrared spectra of cells found in human urine in an effort to develop automatic methods for bladder cancer screening. Unsupervised multivariate analysis of the observed spectral patterns reveals distinct spectral classes, which correlated very well with visual cytology. Therefore, we believe that spectral analysis of individual cells can aid cytology in rendering reliable diagnoses based on objective measurements and discriminant algorithms.

