Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Recombinant DNA

Recombinant DNA

Production of Pharmaceuticals

Production of Pharmaceuticals

Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under sterile, tightly...

The Central Dogma

The Central Dogma

The central dogma explains the flow of genetic information from DNA nucleotides to the amino acid sequence of proteins.
RNA is the Missing Link Between DNA and Proteins
In the early 1900s, scientists discovered that DNA stores all the information needed for cellular functions and that proteins perform most of these functions. However, the mechanisms of converting genetic information into functional proteins remained unknown for many years. Initially, it was believed that a single gene is...

Production of Biopesticides

Production of Biopesticides

Biopesticides offer a sustainable alternative to chemical pesticides, utilizing microbial agents to control agricultural pests. Bacillus thuringiensis (Bt) is a widely employed bacterium known for its potent insecticidal activity. Bt biopesticides are favored for their specificity to insect pests, minimal environmental impact, and natural degradability.Mechanism of Bt Toxin Action Bt produces insecticidal crystal (Cry) proteins during its sporulation phase. These proteins form parasporal...

Transgenic Organisms

Transgenic Organisms

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Assessment of CD8<sup>+</sup> T-cell mediated immunity in an influenza A(H3N2) human challenge model in Belgium: a single centre, randomised, double-blind phase 2 study.

The Lancet. Microbe·2024

Same author

Efficacy of a monovalent (D614) SARS-CoV-2 recombinant protein vaccine with AS03 adjuvant in adults: a phase 3, multi-country study.

EClinicalMedicine·2023

Same author

Efficacy of a bivalent (D614 + B.1.351) SARS-CoV-2 recombinant protein vaccine with AS03 adjuvant in adults: a phase 3, parallel, randomised, modified double-blind, placebo-controlled trial.

The Lancet. Respiratory medicine·2023

Same author

Efficacy of a bivalent (D614 + B.1.351) SARS-CoV-2 Protein Vaccine.

medRxiv : the preprint server for health sciences·2022

Same author

Final Analysis of Efficacy and Safety of Single-Dose Ad26.COV2.S.

The New England journal of medicine·2022

Same author

Safety and immunogenicity of an AS03-adjuvanted SARS-CoV-2 recombinant protein vaccine (CoV2 preS dTM) in healthy adults: interim findings from a phase 2, randomised, dose-finding, multicentre study.

The Lancet. Infectious diseases·2022

Search research articles

Related Experiment Video

Updated: Jun 20, 2026

Transient Expression and Cellular Localization of Recombinant Proteins in Cultured Insect Cells

Transient Expression and Cellular Localization of Recombinant Proteins in Cultured Insect Cells

Published on: April 20, 2017

Recombinant proteins produced in insect cells.

¹Infectious Diseases Division, Department of Medicine, University of Rochester Medical Center, 601 Elmwood Avenue, Rochester, NY 14642, USA. John_Treanor@urmc.rochester.edu

Current Topics in Microbiology and Immunology

|September 22, 2009

Summary

Recombinant influenza vaccines, including hemagglutinin proteins and virus-like particles, show promise. Clinical trials demonstrate safety, efficacy against seasonal flu, and potential for pandemic preparedness.

More Related Videos

Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells

Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells

Published on: August 20, 2018

A Comparative Analysis of Recombinant Protein Expression in Different Biofactories: Bacteria, Insect Cells and Plant Systems

A Comparative Analysis of Recombinant Protein Expression in Different Biofactories: Bacteria, Insect Cells and Plant Systems

Published on: March 23, 2015

Related Experiment Videos

Last Updated: Jun 20, 2026

Transient Expression and Cellular Localization of Recombinant Proteins in Cultured Insect Cells

Transient Expression and Cellular Localization of Recombinant Proteins in Cultured Insect Cells

Published on: April 20, 2017

Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells

Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells

Published on: August 20, 2018

A Comparative Analysis of Recombinant Protein Expression in Different Biofactories: Bacteria, Insect Cells and Plant Systems

A Comparative Analysis of Recombinant Protein Expression in Different Biofactories: Bacteria, Insect Cells and Plant Systems

Published on: March 23, 2015

Area of Science:

Immunology
Vaccinology
Molecular Biology

Background:

Recombinant baculovirus expression systems are utilized for producing influenza vaccine candidates.
Purified proteins and virus-like particles are being investigated as alternatives to traditional vaccines.

Purpose of the Study:

To evaluate the safety and immunogenicity of recombinant influenza vaccines.
To assess the protective efficacy of these vaccines against seasonal and pandemic influenza strains.

Main Methods:

Clinical trials involving healthy and elderly adults.
Administration of recombinant hemagglutinin (rH) vaccines, including a pandemic H5 formulation (rH5).
Assessment of antibody response, tolerability, and protection against influenza.

Main Results:

Recombinant hemagglutinin vaccines were well tolerated and induced functional antibody responses.
Protection against seasonal influenza was observed in adults.
The rH5 vaccine demonstrated neutralizing antibody rates comparable to egg-derived vaccines and showed potential for priming booster responses.

Conclusions:

Recombinant influenza vaccines are safe and effective for seasonal influenza.
Recombinant approaches hold significant potential for future pandemic influenza preparedness.
Further human studies of recombinant pandemic vaccines are warranted.