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Updated: Jun 20, 2026

Live Cell Imaging of Microtubule Cytoskeleton and Micromechanical Manipulation of the Arabidopsis Shoot Apical Meristem
Published on: May 23, 2020
Strategies for imaging microtubules in plant cells
Andreas Holzinger1, Eiko Kawamura, Geoffrey O Wasteneys
1Institute of Botany, Department of Physiology and Cell Physiology, University of Innsbruck, Innsbruck, Austria.
Abstract:
Microtubules are required throughout plant development for a wide variety of processes, and different strategies have been evolved to visualize them. This chapter summarizes the most effective of these methods and points out potential problems and pitfalls. We outline the freeze-shattering method for immunolabeling microtubules in aerial organs such as leaves that require mechanical permeabilization, discuss current options for live cell imaging of MTs with fluorescently tagged proteins (FPs), and provide different fixation protocols for preserving MTs for transmission electron microscopy including chemical fixation, high pressure freezing/freeze substitution, and post-fixation staining procedures for transmission electron microscopy.
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