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Related Concept Videos

Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Jun 20, 2026

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
11:26

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

Scanning probe and nanopore DNA sequencing: core techniques and possibilities.

John Lund1, Babak A Parviz

  • 1Department of Electrical Engineering, University of Washington Paul Allen Center, Seattle, WA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|September 22, 2009
PubMed
Summary

This study reviews DNA sequencing methods using nanopore and scanning probe technologies. It also details the creation of experimental platforms for these advanced DNA studies.

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Last Updated: Jun 20, 2026

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
11:26

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Published on: June 3, 2019

Nanopore DNA Sequencing for Metagenomic Soil Analysis
07:33

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Published on: December 14, 2017

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10:34

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Published on: March 15, 2019

Area of Science:

  • Biotechnology
  • Nanotechnology
  • Molecular Biology

Background:

  • DNA sequencing is crucial for genomics and personalized medicine.
  • Nanopore and scanning probe techniques offer potential for high-resolution DNA analysis.
  • Current research focuses on advancing these novel sequencing methodologies.

Purpose of the Study:

  • To review the current research landscape of DNA sequencing using nanopore and scanning probe techniques.
  • To present methods for constructing key experimental platforms for nanopore and scanning probe DNA studies.

Main Methods:

  • Overview of existing nanopore and scanning probe DNA sequencing research.
  • Development of a synthetic nanopore apparatus.
  • Fabrication of an atomically flat conductive substrate for DNA stretching.

Main Results:

  • Established a comprehensive review of current sequencing technologies.
  • Successfully created a functional synthetic nanopore system.
  • Developed a substrate enabling controlled stretching of DNA molecules for analysis.

Conclusions:

  • The presented platforms facilitate further research in nanopore and scanning probe DNA sequencing.
  • Advancements in these techniques promise more efficient and accurate DNA analysis.
  • This work supports the development of next-generation sequencing technologies.