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Related Concept Videos

Transgenic Plants02:50

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
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Plant Breeding and Biotechnology

Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.

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Related Experiment Video

Updated: Jun 20, 2026

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes
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Genetic transformation of switchgrass.

Yajun Xi1, Yaxin Ge, Zeng-Yu Wang

  • 1Northwest A and F University, Yangling, Shaanxi, China.

Methods in Molecular Biology (Clifton, N.J.)
|September 22, 2009
PubMed
Summary

This study presents a protocol for creating genetically modified switchgrass (Panicum virgatum L.) plants. This method uses Agrobacterium tumefaciens to introduce genes, aiding biofuel crop development.

Area of Science:

  • Plant Biotechnology
  • Genetic Engineering
  • Biofuel Crops

Background:

  • Switchgrass (Panicum virgatum L.) is a highly productive warm-season C4 species.
  • It is a key candidate for dedicated biofuel crop development.
  • Efficient genetic modification is crucial for improving switchgrass traits.

Purpose of the Study:

  • To describe a reliable protocol for generating transgenic switchgrass plants.
  • To establish an efficient Agrobacterium tumefaciens-mediated transformation system for switchgrass.

Main Methods:

  • Embryogenic calluses were induced from switchgrass caryopses or inflorescences.
  • Callus explants were co-cultivated with Agrobacterium tumefaciens strain EHA105.
  • Hygromycin phosphotransferase (hph) gene was used as a selectable marker with hygromycin selection.

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Main Results:

  • Agrobacterium-mediated transformation yielded hygromycin-resistant calluses within 5-6 weeks.
  • Regenerated soil-grown transgenic switchgrass plants were obtained approximately 6 months post-transformation.

Conclusions:

  • This protocol enables the generation of transgenic switchgrass.
  • The established method is valuable for advancing switchgrass as a biofuel feedstock.
  • Further research can utilize this protocol for functional genomics and trait improvement.