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Updated: Jun 20, 2026

High-throughput Saccharification Assay for Lignocellulosic Materials
Published on: July 3, 2011
Simultaneous saccharification and fermentation and partial saccharification and co-fermentation of lignocellulosic
Joy Doran-Peterson1, Amruta Jangid, Sarah K Brandon
1Microbiology Department, University of Georgia, Athens, GA, USA.
Abstract:
Ethanol production by fermentation of lignocellulosic biomass-derived sugars involves a fairly ancient art and an ever-evolving science. Production of ethanol from lignocellulosic biomass is not avant-garde, and wood ethanol plants have been in existence since at least 1915. Most current ethanol production relies on starch- and sugar-based crops as the substrate; however, limitations of these materials and competing value for human and animal feeds is renewing interest in lignocellulose conversion. Herein, we describe methods for both simultaneous saccharification and fermentation (SSF) and a similar but separate process for partial saccharification and cofermentation (PSCF) of lignocellulosic biomass for ethanol production using yeasts or pentose-fermenting engineered bacteria. These methods are applicable for small-scale preliminary evaluations of ethanol production from a variety of biomass sources.
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