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Dry instant blood typing plate for bedside use
D Blakeley1, B Tolliday, C Colaco
1Quadrant Research Foundation, Cambridge Research Laboratories, UK.
Lancet (London, England)
|October 6, 1990
Summary
A novel dry blood grouping plate using trehalose offers indefinite room temperature stability and 99.8% accuracy for ABO and Rhesus blood typing. This innovation supports field and bedside use, especially where refrigeration is unreliable.
Area of Science:
- Biotechnology
- Immunology
- Medical Diagnostics
Background:
- Accurate blood grouping is critical for safe transfusions.
- Current methods often require refrigeration and trained personnel.
- Field and bedside testing limitations hinder timely diagnosis.
Purpose of the Study:
- To develop a stable, room-temperature blood grouping plate.
- To assess the accuracy of a trehalose-based dry plate.
- To enable untrained personnel in field/bedside settings for blood typing.
Main Methods:
- Development of a monoclonal antibody-derived dry plate utilizing trehalose.
- Testing the plate's stability at room temperature.
- Comparative accuracy assessment against a standard semiautomated assay.
Main Results:
- The trehalose-based dry blood grouping plate demonstrated indefinite stability at room temperature.
- The plate achieved 99.8% accuracy when compared to a standard semiautomated assay.
- The plate is suitable for use by personnel without specific training.
Conclusions:
- Trehalose-based dry blood grouping plates offer a stable and accurate solution for ABO and Rhesus typing.
- This technology has significant potential for bedside and field applications.
- It is particularly valuable in resource-limited settings with unreliable refrigeration.