Related Experiment Video
Updated: Jun 20, 2026

The Lambda Select cII Mutation Detection System
Published on: April 26, 2018
The efficiency of induction of mutations by hydroxylamine
J H Phillps1, D M Brown, L Grossman
1University Chemical Laboratory, Cambridge, England.
Abstract:
Treatment of poly C with methoxyamine leads to formation of 5,6-dihydro-6-methoxyaminocytosine residues in the polymer. These direct incorporation of adenylate residues into the poly G synthesized when the modified poly C is used as a template for RNA polymerase. The process is analogous to that of hydroxylamine-induced errors in replication. Comparison of the uptake of [14C]methoxyamine by the poly C with the percentage of adenylate incorporated in the poly G shows that the induction of errors is a highly efficient process: every modified cytosine residue directs the incorporation of one adenylate residue. A probable explanation for this is that the incorporation is mediated by hydrogen-bonding between the adenine and a predominant imino tautomer of the cytosine adduct.
More Related Videos
Related Concept Videos
Spontaneous and Induced Mutations
In vitro Mutagenesis
Mutagenicity and Carcinogenicity
In-vitro Mutagenesis
Aldehydes and Ketones with Amines: Enamine Formation Mechanism
Mismatch Repair
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...

