Related Experiment Video
Updated: Jun 20, 2026

Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells
Published on: May 12, 2023
EIN2, the central regulator of ethylene signalling, is localized at the ER membrane where it interacts with the
Melanie M A Bisson1, Andrea Bleckmann, Silke Allekotte
1Department of Plant Biochemistry, Heinrich-Heine University, 40225 Düsseldorf, Universitätsstrasse 1, Germany.
Abstract:
Genetic studies have identified the membrane protein EIN2 (ethylene insensitive 2) as a central component of ethylene signalling in Arabidopsis. In addition, EIN2 might take part in multiple hormone signalling pathways and in response to pathogens as demonstrated by recent genetic and biochemical studies. Here we show, by an integrated approach using in vivo and in vitro fluorescence techniques, that EIN2 is localized at the ER (endoplasmic reticulum) membrane where it shows specific interaction with the ethylene receptor protein ETR1.
Related Concept Videos
Cell Signaling in Plants
Regulation of the Unfolded Protein Response
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal
The Unfolded Protein Response
Protein Translocation Machinery on the ER Membrane
Sec61 protein conducting channel
In eukaryotes, the translocon complex comprises a core heterotrimeric translocator channel called the Sec61 complex. This channel includes three transmembrane proteins, Sec61α, Sec61β, and Sec61γ, and is the largest subunit of the translocon complex.
Assembly of the Lipid Bilayer in the ER
A large chunk of any biological membrane is composed of phospholipids. These lipids have a heterogeneous distribution across different subcellular organelles and even between...

