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Published on: November 8, 2017
Effect of albumin concentration and serum matrix on ischemia-modified albumin
Aylin Hakligör1, Arzu Kösem, Mehmet Seneş
1Department of Clinical Biochemistry, Ankara Training and Research Hospital, Ulucanlar Caddesi, Cebeci, Ankara 06340, Turkey.
Objectives:
There is concern that ischemia-modified albumin (IMA) levels measured by albumin cobalt binding (ACB) assay reflect mainly albumin concentrations rather than myocardial ischemia.
Design And Methods:
Serum matrix and proteins were separated from a serum pool by a membrane filter. Two series of pools with albumin concentrations of 10, 20, 30, 40, 50, and 60 g/L were prepared either with human albumin or serum protein fraction. IMA values of these pools were measured in quintiplicate.
Results:
There was a strong negative correlation between IMA and albumin levels in both pools. IMA change corresponding to each 10 g/L difference in albumin concentration was 37% and 48% in these pools.
Conclusions:
ACB assay reflects albumin concentrations rather than IMA. Primary predictor of IMA in serum matrix is albumin concentration.
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