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Aspergillus oryzae atfA controls conidial germination and stress tolerance
Kazutoshi Sakamoto1, Kazuhiro Iwashita, Osamu Yamada
1National Research Institute of Brewing, 3-7-1 Kagamiyama, Higashi-Hiroshima, Hiroshima, Japan. k.sakamoto@nrib.go.jp
Fungal Genetics and Biology : FG & B
|September 23, 2009
Summary
The Aspergillus oryzae atfA gene is crucial for conidia germination and oxidative stress resistance, regulating key metabolic pathways. Unlike atfB, atfA controls essential carbon and nitrogen metabolism for germination.
Area of Science:
- Molecular Biology
- Microbiology
- Genetics
Background:
- Aspergillus oryzae possesses two ATF/CREB-type transcription factors, encoded by atfA and atfB.
- Understanding the distinct roles of these transcription factors is essential for comprehending fungal development and stress response.
Purpose of the Study:
- To compare the functions of atfA and atfB in Aspergillus oryzae.
- To elucidate the role of atfA in conidia germination and stress resistance.
Main Methods:
- Comparative analysis of gene knockout strains (DeltaatfA and DeltaatfB).
- Assessment of conidia germination ratios under various conditions.
- Evaluation of sensitivity to oxidative stress.
- Gene expression profiling using DNA microarray (GeneChip).
Main Results:
- DeltaatfA conidia exhibited low germination without stress and increased sensitivity to oxidative stress compared to DeltaatfB.
- AtfA regulates a broader set of genes than atfB, including those involved in oxidative stress resistance.
- Glutamate levels were significantly reduced in DeltaatfA conidia, and glycerol accumulation during germination was absent.
Conclusions:
- AtfA plays a critical role in Aspergillus oryzae conidia germination, independent of atfB.
- AtfA is involved in regulating carbon and nitrogen source metabolism crucial for germination.
- AtfA's regulatory scope extends beyond atfB, encompassing oxidative stress resistance pathways.
