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Expression of fibroblast growth factor binding protein in head and neck cancer
Weimin Li1, Chuan Wang, Steven K Juhn
1Department of Otolaryngology, University of Minnesota School of Medicine, Minneapolis, MN 55455, USA.
Objectives:
To characterize the expression of fibroblast growth factor binding protein (FGF-BP) messenger RNA (mRNA) in head and neck squamous cell carcinoma (HNSCC) and to study the association of FGF-BP with vascularity.
Design:
The expression of FGF-BP mRNA in HNSCC was studied in 35 primary and 8 metastatic HNSCC specimens and 7 control tissues using in situ hybridization and reverse transcriptase-polymerase chain reaction (RT-PCR). Microvessels in tumor specimens were identified with endothelial cell markers (von Willebrand factor [vWF] and CD34-specific antibodies). Correlates between FGF-BP and microvessel counts were evaluated statistically.
Setting:
University of Minnesota Hospitals and Clinics.
Patients:
Forty-two surgically treated patients with HNSCC.
Interventions:
The patients were routinely treated in the study hospitals and clinics.
Main Outcome Measures:
The expression of FGF-BP and angiogenesis in tumors were evaluated.
Results:
In situ hybridization and RT-PCR demonstrated that FGF-BP mRNA transcripts were expressed in 34 of 35 primary HNSCC specimens and 5 of 8 metastatic tumor specimens but not in adjacent control tissues. The microvessel counts in HNSCC specimens were closely related to the expression level of FGF-BP (P < .001).
Conclusion:
The expression of FGF-BP is statistically linked to the angiogenesis of HNSCC, suggesting that FGF-BP participates in the angiogenesis of HNSCC.
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