Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Types of RNA

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...

Types of RNA

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

Regulation of Expression at Multiple Steps

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Maintenance of complex I and its supercomplexes by NDUF-11 is essential for mitochondrial structure, function and health.

Journal of cell science·2021

Same author

Deficiency of the zinc finger protein ZFP106 causes motor and sensory neurodegeneration.

Human molecular genetics·2015

Same author

A novel SOD1-ALS mutation separates central and peripheral effects of mutant SOD1 toxicity.

Human molecular genetics·2014

Same author

Novel mutations in human and mouse SCN4A implicate AMPK in myotonia and periodic paralysis.

Brain : a journal of neurology·2014

Same author

Carboxy terminus of GATA4 transcription factor is required for its cardiogenic activity and interaction with CDK4.

Mechanisms of development·2014

Same author

RAD-6: pyrimidine synthesis and radiation sensitivity in Caenorhabditis elegans.

The Biochemical journal·2013

Search research articles

Related Experiment Video

Updated: Jun 20, 2026

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation

Published on: June 21, 2016

Manipulating and enhancing the RNAi response.

Peter I Joyce¹, Joseph M Gallagher, Patricia E Kuwabara

¹Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol, BS8 1TD, UK.

Journal of Rnai and Gene Silencing : an International Journal of RNA and Gene Targeting Research

|September 23, 2009

Summary

RNA mediated interference (RNAi), first seen in C. elegans, is a powerful tool for gene function annotation. Research in worms has identified key genes that enhance RNAi efficiency in other organisms.

Keywords:

C. elegans RNA interference RdRP siRNA systemic RNAi

More Related Videos

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation

Published on: February 1, 2019

Related Experiment Videos

Last Updated: Jun 20, 2026

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation

Published on: June 21, 2016

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation

Published on: February 1, 2019

Area of Science:

Genetics
Molecular Biology
Biochemistry

Background:

RNA mediated interference (RNAi) was initially discovered in the nematode C. elegans.
The mechanisms of RNAi have been elucidated through genetic and biochemical studies.
RNAi is a widely adopted technology with applications in various research fields.

Purpose of the Study:

To review the application of RNAi in C. elegans for gene function annotation.
To highlight the identification of genes that modulate the RNAi pathway in C. elegans.
To discuss the potential for applying C. elegans RNAi insights to other organisms.

Main Methods:

Utilizing high-throughput analysis and screening protocols in C. elegans.
Leveraging whole genome sequences for RNAi-based gene annotation.
Conducting genetic screens to identify RNAi-essential or modulating genes.

Main Results:

RNAi has proven effective for high-throughput screening and gene function annotation in C. elegans.
Genetic screens have identified crucial genes involved in the RNAi pathway.
Conserved genes identified in C. elegans offer opportunities to enhance RNAi in other species.

Conclusions:

C. elegans serves as a valuable model for understanding and optimizing RNAi.
Insights from C. elegans research can significantly improve RNAi efficiency and potency in diverse biological systems.
The conserved nature of RNAi pathway genes facilitates cross-species application of research findings.