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Related Experiment Video

Updated: Jun 20, 2026

Effect of Fluorescent Proteins on Fusion Partners Using Polyglutamine Toxicity Assays in Yeast

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Published on: November 28, 2018

The structure and function of fluorescent proteins.

Vedangi Sample¹, Robert H Newman, Jin Zhang

¹Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

Chemical Society Reviews

|September 23, 2009

Summary

This summary is machine-generated.

Fluorescent proteins (FPs) offer diverse colors for live cell imaging. Their properties depend on molecular factors and microenvironment, enabling advanced biological research applications.

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Area of Science:

Biochemistry
Molecular Biology
Cell Biology

Background:

Fluorescent proteins (FPs) are crucial tools in live cell imaging, offering a wide spectrum of colors.
FPs generate their own light-emitting chromophores via autocatalytic posttranslational modifications.
The photophysical properties of GFP-family proteins are sensitive to their surrounding microenvironment.

Purpose of the Study:

To review the molecular factors influencing fluorescent protein photophysical properties.
To discuss the applications of fluorescent proteins in live cell imaging.

Main Methods:

Examination of molecular determinants of FP photophysics.
Review of FP applications in live cell imaging.

Main Results:

FP color variants span the visible spectrum, providing versatile imaging probes.
Autocatalytic chromophore formation is a key feature of FP maturation.
Microenvironmental factors significantly modulate FP fluorescence characteristics.

Conclusions:

Understanding molecular factors is key to optimizing FP probes for live cell imaging.
FPs are indispensable for advancing live cell imaging techniques and biological discovery.