Related Experiment Video
Updated: Jun 20, 2026

Isolation and Analysis of Microbial Communities in Soil, Rhizosphere, and Roots in Perennial Grass Experiments
Published on: July 24, 2018
NifH-based studies on azotobacterial diversity in cotton soils of India
Ranjana Bhatia1, Silke Ruppel, Neeru Narula
1Department of Microbiology, College of Basic Sciences and Humanities CCS Haryana Agricultural University, Hisar, 125004, India. ranjanabhatia20@yahoo.com
Abstract:
In order to promote the use of Azotobacter inoculants for cotton crop, a complete characterization of soil isolates of Azotobacter, isolated and screened on the basis of physiological properties, from four different cotton-wheat cropping regions of India was carried out, and their genetic diversity determined by RFLP (restriction fragment length polymorphism) analysis of the functional gene nifH. Genetic analysis of these isolates depicted a similarity coefficient of > or = 80% among them, suggesting that though the isolates were obtained from different cotton soils of India, still they have large commonality in the nifH gene and constituted a homogeneous nifH population.
Related Concept Videos
The Roles of Bacteria and Fungi in Plant Nutrition
Microbes and the Nitrogen Cycle
Microorganisms in Agriculture and Food industry
Inorganic Nitrogen Assimilation
Soil Microbial Ecology
Overview of Nitrogen Metabolism
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this nitrogen...

