[Regulation of protein kinases on signal pathway in breast cancer cell MCF-7 by curcumin]
Related Experiment Video
Updated: Jun 20, 2026

Exploring the Pharmacological Action and Molecular Mechanism of Salidroside in Inhibiting MCF-7 Cell Proliferation and Migration
Published on: June 9, 2023
Qian-Mei Zhou1, Shi-Bing Su, Hui Zhang
1Shanghai University of TCM, Shanghai 201203, China.
Objective:
To observe the effect of curcumin on protein kinases on cell signal pathway in breast cancer cell MCF-7.
Methods:
The suppressive effect of curcumin on MCF-7 cell proliferation as different concentration and at different time points were observed by MTT assay; The activity of p-ERK, p-NF-kappaB, p-p38 and p-JNK were observed by western blot.
Results:
The suppressive effect of curcumin was shown as a dose dependent and a time dependent manner,and the IC50 was 22.48 micromol/L 40 micromol/L curcumin inhibited the expression of p-ERK, p-NF-kappaB and p-p38, but not p-JNK.
Conclusion:
Curcumin can inhibit MCF-7 cell's proliferation, and its mechanism may be related to the activities of protein kinases on cell signal pathway.
Related Concept Videos
mTOR Signaling and Cancer Progression
The mTOR pathway or the...
mTOR Signaling and Cancer Progression
The mTOR pathway or the...
PI3K/mTOR/AKT Signaling Pathway
Interactions Between Signaling Pathways
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Inhibition of Cdk Activity
Inhibition of CDK Activity